Journal:

Article Title: Trauma-hemorrhagic shock-induced pulmonary epithelial and endothelial cell injury utilizes different programmed cell death signaling pathways

doi: 10.1152/ajplung.00491.2007

Figure Lengend Snippet: T/HS induces alveolar epithelial cell apoptosis via caspase-3-dependent pathway. A: caspase-3 immunohistochemical staining of lung tissue from rat subjected to T/HS. Arrows indicate cleaved caspase-3-positive alveolar epithelial cells. Magnification, ×100. B: number of cleaved caspase-3-positive epithelial cells per 100 hpf. Data are shown as mean values ± SD (n = 4–6 per group). *P < 0.01 vs. T/SS. C: M30 immunohistochemical staining of lung tissue from rats subjected to T/SS or T/HS. Magnification, ×40. D: number of M30-positive alveolar epithelial cells per 40 hpf. Data are shown as mean values ± SD (n = 4–6 per group). *P < 0.001 vs. T/SS.

Article Snippet: Slides were incubated with M30 CytoDeath Biotin (DiaPharma, Columbus, OH) at 1:50 and counterstained with the VectaStain ABC Kit (Vector Laboratories, Burlingame, CA) as previously described ( 42 ).

Techniques: Immunohistochemical staining, Staining

Journal:

Article Title: Trauma-hemorrhagic shock-induced pulmonary epithelial and endothelial cell injury utilizes different programmed cell death signaling pathways

doi: 10.1152/ajplung.00491.2007

Figure Lengend Snippet: Inhibition of caspases by zVAD protects against T/HS lymph-induced A549 cell apoptosis. A: A549 cells were exposed to 5% T/HS and 5% T/SS lymph for 3 h, immunostained with M30 (green), and examined by immunofluorescence. The nuclei were stained with PI (red). B: the mean values ± SD shown in the bar graph for the number of M30 apoptotic A549 cells counted per 100 cells. *P < 0.001 vs. T/SS lymph. C: A549 cells were pretreated with zVAD (100 μM) or DMSO vehicle control for 1 h and then exposed to medium, STS (1 μM), and 5% T/HS and 5% T/SS lymph for 3 h. Cell death was assessed by MTT cell viability assay. In the bar graph, the data are expressed as mean values ± SD for percent cell viability. *P < 0.01 STS vs. STS, and **P < 0.01 T/HS vs. T/HS and zVAD (n = 6–8 per condition).

Article Snippet: Slides were incubated with M30 CytoDeath Biotin (DiaPharma, Columbus, OH) at 1:50 and counterstained with the VectaStain ABC Kit (Vector Laboratories, Burlingame, CA) as previously described ( 42 ).

Techniques: Inhibition, Immunofluorescence, Staining, Control, Viability Assay

Journal: Oncogene

Article Title: Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death

doi: 10.1038/onc.2015.348

Figure Lengend Snippet: (A) LC3-II accumulation and P-ERK levels in NSCLC cells treated with chloroquine (CQ) or quinacrine (Q) for 18 h at indicated doses. (B, C) Growth curves of NSCLC cells treated with CQ ( b ) and Q ( c ) at indicated doses. (D) Growth curves following ATG7 depletion and corresponding immunoblots of LC3-II reduction and ATG7 knockdown. ( E , F ) Q but not CQ reduces NSCLC cell survival. Cells were treated with 30μM CQ or 10μM Q for 24h. Immunoblotting for cleaved PARP and ELISA for cleaved cytokeratin 18 (cCK18) confirms that Q promotes apoptosis. UT=untreated, STS= 1μM staurosporine (positive control). All data represent mean ± SEM from 3 independent experiments. Statistical significance was calculated using Student’s t-test for pair-wise comparisons in (B) or by ANOVA followed by Tukey’s HSD. *P≤0.05, **P≤0.01, ***P≤0.001.

Article Snippet: The caspase cleaved cytokeratin 18 (cCK18) M30 fragment was measured using the M30 Cytodeath ELISA (Diapharma P10900), according to the manufacturer’s protocol; this assay quantitatively measures a stable apoptosis-generated neoepitope in CK-18 (Asp396).

Techniques: Western Blot, Knockdown, Enzyme-linked Immunosorbent Assay, Positive Control

Journal: Oncogene

Article Title: Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death

doi: 10.1038/onc.2015.348

Figure Lengend Snippet: (A, B) Cells were treated with 30μM CQ or 10μM Q treatment for 6h. Reactive oxygen species (ROS) production in A549 ( A ) or H460 ( B ) lung cancer cells was measured using a bioluminescence assay to detect intracellular H 2 O 2 levels; results were normalized to those obtained with untreated controls (UT). (C, D) A549 ( C ) or H460 ( D ) cells were treated with 30μM CQ or 10μM Q for 24h and cleaved cytokeratin 18 (cCK18) levels were measured using ELISA. UT=untreated controls. NAC= 5 μM N-Acetyl Cysteine. All data represent the mean ± SEM from 3 independent experiments. Statisitical significance was calculated using ANOVA followed by Tukey’s HSD. *P≤0.05, **P≤0.01, ***P≤0.001.

Article Snippet: The caspase cleaved cytokeratin 18 (cCK18) M30 fragment was measured using the M30 Cytodeath ELISA (Diapharma P10900), according to the manufacturer’s protocol; this assay quantitatively measures a stable apoptosis-generated neoepitope in CK-18 (Asp396).

Techniques: ATP Bioluminescent Assay, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Zika virus replication and cytopathic effects in liver cells

doi: 10.1371/journal.pone.0214016

Figure Lengend Snippet: Measurement of Zika NS1 protein in target cell line supernatant over time, by ELISA. Results are expressed as the log ng/mL of the mean of 2–4 replicates in two experiments. Bars represent standard error of the mean. Asterisks denote data with statistically significant change from baseline.

Article Snippet: We utilized the M30 CytoDeath ELISA (Diapharma, West Chester, OH) to detect and quantify apoptosis in the cell cultures after exposure to ZIKV over time.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Zika virus replication and cytopathic effects in liver cells

doi: 10.1371/journal.pone.0214016

Figure Lengend Snippet: Determination of apoptosis in undiluted cell supernatants over time by M30 CytoDeath ELISA (enzyme-linked immunosorbent assay). Results are the mean of 2–4 replicates, bars representing standard error of the mean. Assay detection limit is 60–3000 U/L; LLOQ is 250 U/L. Asterisk denotes data with statistically significant changes from baseline.

Article Snippet: We utilized the M30 CytoDeath ELISA (Diapharma, West Chester, OH) to detect and quantify apoptosis in the cell cultures after exposure to ZIKV over time.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: SpringerPlus

Article Title: Placental abruption possibly due to parvovirus B19 infection

doi: 10.1186/s40064-016-2946-2

Figure Lengend Snippet: Immunohistochemical findings of all placentas using M30 CytoDEATH antibody. Immunostaining for apoptosis in case 1 a, b showed positive findings in the decidual and contiguous chorionic cells ( arrows ), while the three control cases, including case 2 c, d , showed no staining of the chorionic cells. e Graph, showing the percentage of M30-positive decidual and chorionic cells in each case

Article Snippet: Accordingly, we performed a quantitative polymerase chain reaction (PCR) test (Umene and Nunoue ) for PB19-specific DNA to determine the presence of infection with microscopic sections of placenta and confirmed apoptosis of the decidual and chorionic sites using immunostaining with M30 CytoDEATH antibody (M30 Mab; VLVbio AB, Stockholm, Sweden), which identifies cytokeratin 18 fragmented due to apoptosis (Jordan and Butchko ; Abumaree et al. ).

Techniques: Immunohistochemical staining, Immunostaining, Control, Staining